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Chart: (FYI open circles are due to a varied schedule/wake up time...next cycle I will try the 4am-temp-then-back-to-sleep approach!) Reasons I am. Step 1: Before ovulation, estradiol (E2) and luteinizing hormone (LH) run the show. In the days before ovulation, there are two hormones that primarily run the show: estradiol (E2) and luteinizing hormone (LH). All about E2: A couple of days before ovulation during an average cycle, there is one growing, thriving follicle which houses the egg.